Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 2 Peptide 3
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG2a, kappa light chain
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antigen is also known as RAC-beta serine/threonine-protein kinase.
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: 55.7 kDa
Immunogen: Synthetic peptide
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Immunogen Sequence: THFPQFSYSASIRE (C-terminus; a.a. 468-481)
Uniprot ID: P31751
Additional Information: RRID:AB_2722015
Entrez Gene ID: 208
Antibody Registry ID: AB_2722015
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-AKT2-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-AKT2-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).