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View product citations for antibody CPTC-AKT2-6 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: AKT Serine/Threonine Kinase 2 Peptide 3
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Clinical Proteomics Technologies for Cancer
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: National Cancer Institute
Antigen Molecular Weight: 55.7 kDa
Depositors Notes: Ras Pathway
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Phosphorylated synthetic peptide
Gene: AKT2
Alternate Antibody Name:
Alternate Antigen Name:
Clonality: rabbit recombinant monoclonal
Myeloma Strain: 240E-W2
Epitope Mapped: No
Uniprot ID: P31751 
Epitope Location or Sequence: Starting at amino acid 468.
Entrez Gene ID: 208 
Immunogen Sequence: THFPQF(pS)YSASIRE
Recommended Applications: FFPE, Immunohistochemistry, Western Blot
Additional Information: AB_2889034
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-AKT2-6 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-AKT2-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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