CPTC-AML1-ETO AcK43-1
Catalog Fields
Product Name/ID: CPTC-AML1-ETO AcK43-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: RUNX1T1
Ab Isotype: Rabbit IgG
Gene Symbol:
Entrez Gene ID: 862
Clonality: Monoclonal
Immunogen: Acetylated synthetic peptide
Clone:
Immunogen Sequence: CALAG(KAc)LRSGDR
Myeloma Strain: 240E-W2
Epitope Mapped: Yes
Antigen Name: AML1-ETO AcK43 Peptide 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/2/2022
Antigen Molecular Weight: 67.5 kDa
Depositor: Clinical Proteomics Technologies for Cancer
Antigen Sequence:
Depositor Institution: National Cancer Institute
Antigen Species: Human
Depositor Notes: This antibody is specific for acetylated lysine 43 in the AML1-ETO fusion protein sequence. AML1-ETO is generated by genetic translocation in acute myelogenous leukemia. See PMID: 21764752
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information:
Additional Characterization: https://antibodies.cancer.gov/detail/AML1-ETOAcK43Peptide1
Recommended Applications:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-AML1-ETO AcK43-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-AML1-ETO AcK43-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
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