Antigen: Breast Cancer 1, Early Onset Peptide 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This monoclonal antibody recognizes non-phosphorylated CPTC-BRCA1 peptide 1. This antigen is also known as breast cancer type 1 susceptibility protein
Antigen Molecular Weight: 63.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Synthetic peptide
Alternate Gene Names: Breast Cancer 1, Early Onset; RNF53; RING Finger Protein 53; BROVCA1; PNCA4; PSCP; BRCA1/BRCA2-Containing Complex; Protein Phosphatase 1; Regulatory Subunit 53; Subunit 1; BRCAI; BRCC1; IRIS; PPP1R53; BRCA1/BRCA2-Containing Complex, Subunit 1; Breast And Ovarian Cancer Susceptibility Protein1; Breast Cancer Type 1 Susceptibility Protein; Protein Phosphatase 1, Regulatory Subunit 53; EC 6.3.2.
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: a.a. 1520-1531
Uniprot ID: P38398
Immunogen Sequence: RNYP(pS)QEELIKV
Antibody Registry ID: AB_2722030
Additional Information: RRID:AB_2722030
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-BRCA1-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-BRCA1-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).