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View product citations for antibody CPTC-BRCA1-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: CPTC-BRCA1-2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: BRCA1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2722030 
Uniprot ID: P38398 
Entrez Gene ID: 672 
Clonality: Monoclonal
Immunogen: Synthetic peptide
Immunogen Sequence: RNYPSQEELIKV
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: Breast Cancer 1, Early Onset Peptide 1
Epitope Location or Sequence: a.a. 1520-1531
Alternate Antigen Name:
Deposit Date: 5/19/2015
Antigen Molecular Weight: 63.6 kDa
Depositor: Clinical Proteomics Technologies for Cancer
Antigen Sequence:
Depositor Institution: National Cancer Institute
Antigen Species: Human
Depositor Notes: This monoclonal antibody recognizes non-phosphorylated CPTC-BRCA1 peptide 1. This antigen is also known as breast cancer type 1 susceptibility protein
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2722030
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-BRCA1-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-BRCA1-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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