Antigen: CD74 Molecule Peptide 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Paulovich, A.P.
Isotype: Rabbit IgG
Host Species: rabbit
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as HLA class II histocompatibility antigen gamma chain which stabilizes MHC class.
Predicted Species Reactivity:
Immunogen: Synthetic Peptide
Alternate Gene Names: DHLAG, HLADG, II, Ia-GAMMA, p33
Alternate Antibody Name:
Clonality: rabbit recombinant monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: 240E-W2
Epitope Location or Sequence: a.a. 212-213
Uniprot ID: P04233
Immunogen Sequence: CQEEVSHIPAVHPGSFRPK (Initial Cys in carbamidomethylated)
Entrez Gene ID: 972
Additional Characterization: https://antibodies.cancer.gov/detail/CD74MoleculePeptide1#CPTC-CD74-1
Antibody Registry ID: AB_2889054
Additional Information: This antibody has been validated for IHC and IF by the HPA (#CAB079994).
Recommended Applications: Immuno-MRM, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-CD74-1 was deposited to the DSHB by Paulovich, A.P. (DSHB Hybridoma Product CPTC-CD74-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).