Product Name/ID: CPTC-CD74-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CD74
Ab Isotype: Rabbit IgG
Entrez Gene ID: 972
Clonality: rabbit recombinant monoclonal
Immunogen: Synthetic Peptide
Immunogen Sequence: CQEEVSHIPAVHPGSFRPK (Initial Cys in carbamidomethylated)
Myeloma Strain: 240E-W2
Epitope Mapped: No
Antigen Name: CD74 Molecule Peptide 1
Epitope Location or Sequence: a.a. 212-213
Alternate Antigen Name:
Deposit Date: 12/15/2020
Antigen Molecular Weight: 33.5 kDa
Depositor: Paulovich, A.P.
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: This antigen is also known as HLA class II histocompatibility antigen gamma chain which stabilizes MHC class.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: This antibody has been validated for IHC and IF by the HPA (#CAB079994).
Additional Characterization: https://antibodies.cancer.gov/detail/CD74MoleculePeptide1#CPTC-CD74-1
Recommended Applications: Immuno-MRM, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-CD74-1 was deposited to the DSHB by Paulovich, A.P. (DSHB Hybridoma Product CPTC-CD74-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).