CPTC-CHEK1-3

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$40.00
SKU: CPTC-CHEK1-3-s
View product citations for antibody CPTC-CHEK1-3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Checkpoint Kinase 1 peptide 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG2c
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antigen is also known as serine/threonine-protein kianse Chk1.
Positive Tested Species Reactivity: Human
Gene: CHEK1
Antigen Molecular Weight: 54.4 kDa
Alternate Gene Names: CHK1; Checkpoint Kinase 1; Cell Cycle Checkpoint Kinase; Checkpoint Kinase-1; CHK1 Checkpoint Homolog; CHK1 (Checkpoint, S.Pombe) Homolog; Chk1-S; Checkpoint, S. Pombe, Homolog Of; Serine/Threonine-Protein Kinase Chk1
Immunogen: Synthetic Peptide
Clonality: Monoclonal
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: No
Uniprot ID: O14757 
Epitope Location or Sequence: a.a. 312-323
Entrez Gene ID: 1111 
Immunogen Sequence: CKYSS(pS)QPEPRT
Antibody Registry ID: AB_2722046 
Recommended Applications: ELISA
Additional Information: RRID:AB_2722046
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-CHEK1-3 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-CHEK1-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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