Antigen: Deleted in Liver Cancer 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes the delieted in liver cancer 1 protein, a GTPase-activating protein that participates in GAP family signaling pathways involved in cytoskeletal changes.
Antigen Molecular Weight: 26 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant Full Length Protein
Alternate Gene Names: Deleted In Liver Cancer 1 Variant 2; ARHGAP7; STARD12; HP; P122-RhoGAP; DLC-1; Deleted In Liver Cancer 1 Protein; Rho-Type GTPase-Activating Protein 7; START Domain-Containing Protein 12; Rho GTPase-Activating Protein 7; StAR-Related Lipid Transfer (START) Domain Containing 12; KIAA1723; StARD12; HP Protein; StAR-Related Lipid Transfer Protein 12
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q96QB1
Immunogen Sequence: Full length
Antibody Registry ID: AB_2617248
Additional Information: RRID:AB_2617248
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-DLC1-3 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-DLC1-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).