Product Name/ID: CPTC-FOXO3-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: FOXO3
Ab Isotype: Rabbit IgG
Entrez Gene ID: 2309
Immunogen: Synthetic Peptide
Immunogen Sequence: SPAKPSGETAADSMIPEEEc
Myeloma Strain: 240E-W2
Epitope Mapped: No
Antigen Name: Forkhead Box O3 Peptide 1
Epitope Location or Sequence: a.a. 43-61
Alternate Antigen Name:
Deposit Date: 3/9/2019
Antigen Molecular Weight: 71.28 kDa
Depositor: Clinical Proteomics Technologies for Cancer
Depositor Institution: National Cancer Institute
Antigen Species: Human
Depositor Notes: This antigen functions as a trigger for apoptosis.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: RRID: AB_2827869
Additional Characterization: https://antibodies.cancer.gov/detail/ForkheadBoxO3Peptide1
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-FOXO3-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-FOXO3-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.