Antigen: Heat shock 60kDa protein 1 (chaperonin)
Depositor: Clinical Proteomics Technologies for Cancer
Antigen Species: Human
Depositors Institution: National Cancer Institute
Host Species: mouse
Alternate Gene Names: Heat shock 60kDa protein 1 (chaperonin); HSP60; GROEL; Mitochondrial matrix protein P1; Chaperonin 60; HuCHA60; P60 lymphocyte protein; HSP-60; 60 kDa chaperonin; CPN60; SPG13; HLD4; heat shock 60kD protein 1; spastic paraplegia 13; 60 kDa heat shock protein, mitochondrial; short heat shock protein 60 Hsp60s1; HSP65; heat shock protein 65; Heat shock protein 60; Hsp60;
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: 61.3 kDa
Additional Characterization: https://antibodies.cancer.gov/detail/CPTC-HSPD1-1
Immunogen: Recombinant Full Length Protein
Additional Information: This antibody has been characterized only by ELISA or western blot. RRID:AB_2617272
Myeloma Strain: P3x63Ag8.653
Uniprot ID: P10809
Entrez Gene ID: 3329
Antibody Registry ID: AB_2617272
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-HSPD1-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-HSPD1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).