CPTC-MAPK3-2

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$40.00
SKU: CPTC-MAPK3-2-s
View product citations for antibody CPTC-MAPK3-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Mitogen-Activated Protein Kinase 3 Peptide 2
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG1, kappa light chain
Depositors Institution: National Cancer Institute
Host Species: mouse
Gene: Erk1
Positive Tested Species Reactivity: Human
Alternate Gene Names: ERK1, p44erk1, p44mapk, PRKM3
Antigen Molecular Weight: 43.1 kDa
Clonality: Monoclonal
Immunogen: Synthetic peptide ALDLLDR
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Immunogen Sequence: a.a. 303-309
Uniprot ID: P27361 
Additional Information: RRID:AB_2722081; RAS pathway related proteins
Entrez Gene ID: 5595 
Antibody Registry ID: AB_2722081 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-MAPK3-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-MAPK3-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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