Antigen: neural precursor cell expressed, developmentally down-regulated 4 isoform 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Depositors Institution: National Cancer Institute
Host Species: mouse
Positive Tested Species Reactivity: Human
Alternate Gene Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitinprotein ligase; NEDD4-1; RPF1; KIAA0093; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4; MGC176705; neural precursor cell expressed, developmentally down-regulated 4; E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; EC 6.3.2.
Antigen Molecular Weight: 105.8 kDa
Immunogen: Recombinant Full Length Protein
Epitope Mapped: No
Myeloma Strain: P3x63Ag8.653
Additional Characterization: https://antibodies.cancer.gov/detail/CPTC-NEDD4-2
Uniprot ID: P46934
Additional Information: Antibody has been characterized only by ELISA or western blot. RRID:AB_2617297
Entrez Gene ID: 4734
Antibody Registry ID: AB_2617297
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-NEDD4-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-NEDD4-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).