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View product citations for antibody CPTC-PSAT1-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Phosphoserine Aminotransferase 1
Depositor: Clinical Proteomics Technologies for Cancer
Antigen Species: Human
Depositors Institution: National Cancer Institute
Isotype: MIgG1
Depositors Notes: This antibody has been validated by the Human Protein Atlas. http://www.proteinatlas.org/ENSG000 00135069?PSAT1/antibody.
Host Species: mouse
Positive Tested Species Reactivity: Human
Gene: PSAT1
Antigen Molecular Weight: 40.7 kDa
Alternate Gene Names: PSAT; PSA; MGC1460; EPIP; EC
Immunogen: Recombinant Full Length Protein
Clonality: Monoclonal
Myeloma Strain: P3x63Ag8.653
Uniprot ID: Q9Y617 
Additional Information: This antibody has been characterized only by ELISA or western blot. RRID:AB_10586639
Entrez Gene ID: 29968 
Antibody Registry ID: AB_10586639 
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-PSAT1-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-PSAT1-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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