Antigen: PTEN/ Phosphatase And Tensin Homolog Peptide 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN.
Antigen Molecular Weight: Isoform 1: 47.16 kDa, Isoform alpha: 64.8 kDa, Isoform 3: 19.79 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Synthetic Peptide
Alternate Gene Names: BZS, MHAM, MMAC1, PTEN1, TEP1
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: p3x63Ag8.653
Epitope Location or Sequence: Isoform 1 a.a. 148-159
Uniprot ID: P60484
Immunogen Sequence: AQEALDFYGEVR (Isoform 1 a.a. 148-159)
Entrez Gene ID: 5728
Antibody Registry ID: AB_2617320
Additional Information: This antibody recognizes all three isoforms of PTEN. Isoform alpha a.a. 321-332 (m.w. 64.8 kDa) Isoform 3 a.a. 133-143 (m.w. 19.79 kDa) RRID:AB_2617320
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-PTEN-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-PTEN-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).