CPTC-RAD18-1

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$40.00
SKU: CPTC-RAD18-1
View product citations for antibody CPTC-RAD18-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Rad18 E3 ubiquitin-protein ligase
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as RAD18 homolog of S. cerevisiae Rad18.
Antigen Molecular Weight: 56.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: RAD18
Immunogen: Synthetic Peptide
Alternate Gene Names: RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18, S. Cerevisiae, Homolog; EC 6.3.2.
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q9NS91 
Immunogen Sequence: RNHLLQFALEp(S)PAKS
Entrez Gene ID: 56852 
Additional Characterization: Link 
Antibody Registry ID: AB_2617325 
Additional Information: RRID:AB_2617325
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-RAD18-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-RAD18-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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