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View product citations for antibody CPTC-RAD18-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Rad18 E3 ubiquitin-protein ligase
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: Clinical Proteomics Technologies for Cancer
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: National Cancer Institute
Antigen Molecular Weight: 56.2 kDa
Depositors Notes: This antigen is also known as RAD18 homolog of S. cerevisiae Rad18.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Synthetic Peptide
Gene: RAD18
Alternate Antibody Name:
Alternate Gene Names: RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18, S. Cerevisiae, Homolog; EC 6.3.2.
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: No
Uniprot ID: Q9NS91 
Epitope Location or Sequence:
Entrez Gene ID: 56852 
Immunogen Sequence: RNHLLQFALEp(S)PAKS
Antibody Registry ID: AB_2617325 
Recommended Applications: ELISA
Additional Information: RRID:AB_2617325
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-RAD18-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-RAD18-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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