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SKU: CPTC-S100A4-2-s
View product citations for antibody CPTC-S100A4-2 on CiteAb

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Available: 76

DSHB Data Sheet

Catalog Fields

Antigen: Metastasin 100 calcium-binding protein A4 (Calvasculin)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: 12 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: S100A4
Immunogen: Recombinant Full Length Protein
Alternate Gene Names: S100A4; 18A2; 42A; CAPL; Calvasculin; FSP1; MTS1; Metastasin; P9KA; PEL98
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P26447 
Immunogen Sequence:
Entrez Gene ID: 6275 
Additional Characterization: Link 
Antibody Registry ID: AB_2077378 
Additional Information: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization. RRID:AB_2077378
Recommended Applications: ELISA, FFPE, Immunohistochemistry, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-S100A4-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-S100A4-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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