CPTC-SVIL-4

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$40.00
SKU: CPTC-SVIL-4 -s
View product citations for antibody CPTC-SVIL-4 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Supervillin
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: Supervillin forms a high-affinity link between the actin CSK and the plasma membrane.
Antigen Molecular Weight: 247 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: SVIL
Immunogen: Recombinant protein domain
Alternate Gene Names: Archvillin/p205/p250
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: 95425 
Immunogen Sequence: N-terminal protein domain including 1-220 amino acid sequence
Entrez Gene ID: 6840 
Additional Characterization:
Antibody Registry ID: AB_2753322 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-SVIL-4 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-SVIL-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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