Antigen: Tumor-associated Calcium Signal Transducer 2
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antibody recognizes a cell surface receptor associated with carcinoma.
Positive Tested Species Reactivity: Human
Human Protein Atlas: http://www.proteinatlas.org/ENSG00000184292-TACSTD2/antibody
Antigen Molecular Weight: 28.3 kDa
Immunogen: Recombinant Domain
Alternate Gene Names: M1S1;EGP1; tumor-associated calcium signal transducer 2; GA7331; TROP2; GP50; GA733-1; cell surface glycoprotein TROP2; EGP-1; epithelial glycoprotein-1; Cell surface glycoprotein Trop-2; gastrointestinal tumor-associated antigen GA7331; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331; 40kD glycoprotein, identified by monoclonal antibody GA733;
Myeloma Strain: P3x63Ag8.653
Uniprot ID: P09758
Epitope Mapped: No
Entrez Gene ID: 4070
Immunogen Sequence: a.a 31-274
Antibody Registry ID: AB_2617349
Additional Characterization: https://antibodies.cancer.gov/apps/site/detail/CPTC-TACSTD2-3#CPTC-TACSTD2-3
Recommended Applications: ELISA, Western Blot
Additional Information: RRID:AB_2617349
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-TACSTD2-3 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-TACSTD2-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).