CPTC-TP53-1

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SKU: CPTC-TP53-1-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: CPTC-TP53-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: TP53
Ab Isotype: Rabbit IgG
Gene Symbol:
Antibody Registry ID: AB_2877664 
Uniprot ID: P04637 
RRID:  
Entrez Gene ID: 7157 
Clonality: Monoclonal
Immunogen: Phosphorylated synthetic peptide
Clone:
Immunogen Sequence: CALPNNTSSS(ph)PQPK
Myeloma Strain: 240E-W2
Epitope Mapped: No
Antigen Name: Tumor Protein P53 Peptide 1
Epitope Location or Sequence: a.a. 307-320
Alternate Antigen Name:
Deposit Date: 2/12/2020
Antigen Molecular Weight: 43.6 kDa
Depositor: CPTC/FHCRC
Antigen Sequence:
Depositor Institution: National Cancer Institute
Antigen Species: Human
Depositor Notes:
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: RRID: AB_2877664
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-TP53-1 was deposited to the DSHB by CPTC/FHCRC (DSHB Hybridoma Product CPTC-TP53-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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