CWK-F12

(0) No Reviews yet
$40.00
SKU: CWK-F12-s
View product citations for antibody CWK-F12 on CiteAb

In Stock

Available: 208

DSHB Data Sheet

Catalog Fields

Antigen: Estrogen receptor beta (ER 2)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Katzenellenbogen, B.S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois
Positive Tested Species Reactivity: Human, Mouse, Porcine, Rat
Depositors Notes: This monoclonal antibody has recently be shown to be specific for ER beta unlike other commonly used anti-ER beta antibodies (see Nelson et al 2017 PMID 27889472).
Antigen Molecular Weight: 59 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ESR2
Immunogen: E2 ligand-binding domain
Alternate Gene Names: Erb, NR3A2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: Q92731 
Immunogen Sequence: a.a. 256-505
Entrez Gene ID: 2100 
Additional Characterization:
Antibody Registry ID: AB_2722105 
Additional Information: RRID: AB_2722105
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CWK-F12 was deposited to the DSHB by Katzenellenbogen, B.S. (DSHB Hybridoma Product CWK-F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells.
    Yager JD
    American journal of physiology. Endocrinology and metabolism 286.6 (2004 Jun): E1011-22.

    WB References

    Human estrogen receptor beta-specific monoclonal antibodies: characterization and use in studies of estrogen receptor beta protein expression in reproductive tissues.
    Katzenellenbogen BS
    Molecular and cellular endocrinology 181.1-2 (2001 Jul 5): 139-50.

    Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
    Carroll JS
    Molecular and cellular endocrinology 440. (2017 Jan 15): 138-150.

    ER beta inhibits proliferation and invasion of breast cancer cells.
    Vignon F
    Endocrinology 142.9 (2001 Sep): 4120-30.

    Interaction of estrogen receptors alpha and beta with estrogen response elements.
    Nardulli AM
    Molecular and cellular endocrinology 181.1-2 (2001 Jul 5): 151-63.

    Molecular cloning of porcine estrogen receptor-beta complementary DNAs and developmental expression in periimplantation embryos.
    Simmen RC
    Biology of reproduction 66.3 (2002 Mar): 760-9.

    Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells.
    Yager JD
    American journal of physiology. Endocrinology and metabolism 286.6 (2004 Jun): E1011-22.

    Mechanisms enforcing the estrogen receptor β selectivity of botanical estrogens.
    Katzenellenbogen BS
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.11 (2013 Nov): 4406-18.

    Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells.
    Craig Jordan V
    The Journal of steroid biochemistry and molecular biology 87.1 (2003 Oct): 47-55.

    ERalpha and ERbeta expression and transcriptional activity are differentially regulated by HDAC inhibitors.
    Lazennec G
    Oncogene 25.12 (2006 Mar 16): 1799-806.

    Maximizing production of estrogen receptor beta with the baculovirus expression system.
    Klinge CM
    BioTechniques 34.2 (2003 Feb): 334-8, 340-3.

    Impact of estrogen receptor beta on gene networks regulated by estrogen receptor alpha in breast cancer cells.
    Katzenellenbogen BS
    Endocrinology 147.10 (2006 Oct): 4831-42.

    Placental expression of estrogen receptor beta and its hormone binding variant--comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells.
    Elder RF
    Reproductive biology and endocrinology : RBandE 1. (2003 Apr 15): 36.

    17 beta-estradiol inhibits cardiac fibroblast growth through both subtypes of estrogen receptor.
    Ouchi Y
    Biochemical and biophysical research communications 311.2 (2003 Nov 14): 454-9.

    Estrogen receptor beta mediates the inhibitory effect of estradiol on vascular smooth muscle cell proliferation.
    Ouchi Y
    Cardiovascular research 59.3 (2003 Sep 1): 734-44.

    ERβ- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis.
    Bulun SE
    Molecular endocrinology (Baltimore, Md.) 28.8 (2014 Aug): 1304-15.

    IHC References
    IP References
    ELISA References
    FFPE References
    FB References
    Epitope Map References
    All References

    Human estrogen receptor beta-specific monoclonal antibodies: characterization and use in studies of estrogen receptor beta protein expression in reproductive tissues.
    Katzenellenbogen BS
    Molecular and cellular endocrinology 181.1-2 (2001 Jul 5): 139-50.

    Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
    Carroll JS
    Molecular and cellular endocrinology 440. (2017 Jan 15): 138-150.

    ER beta inhibits proliferation and invasion of breast cancer cells.
    Vignon F
    Endocrinology 142.9 (2001 Sep): 4120-30.

    Molecular cloning of porcine estrogen receptor-beta complementary DNAs and developmental expression in periimplantation embryos.
    Simmen RC
    Biology of reproduction 66.3 (2002 Mar): 760-9.

    Placental expression of estrogen receptor beta and its hormone binding variant--comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells.
    Elder RF
    Reproductive biology and endocrinology : RBandE 1. (2003 Apr 15): 36.

    Expression of steroid hormone receptors in BRCA1-associated ovarian carcinomas.
    de Fazio A
    Gynecologic oncology 97.1 (2005 Apr): 16-25.

    Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells.
    Yager JD
    American journal of physiology. Endocrinology and metabolism 286.6 (2004 Jun): E1011-22.

    Interaction of estrogen receptors alpha and beta with estrogen response elements.
    Nardulli AM
    Molecular and cellular endocrinology 181.1-2 (2001 Jul 5): 151-63.

    Mechanisms enforcing the estrogen receptor β selectivity of botanical estrogens.
    Katzenellenbogen BS
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.11 (2013 Nov): 4406-18.

    Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells.
    Craig Jordan V
    The Journal of steroid biochemistry and molecular biology 87.1 (2003 Oct): 47-55.

    ERalpha and ERbeta expression and transcriptional activity are differentially regulated by HDAC inhibitors.
    Lazennec G
    Oncogene 25.12 (2006 Mar 16): 1799-806.

    Maximizing production of estrogen receptor beta with the baculovirus expression system.
    Klinge CM
    BioTechniques 34.2 (2003 Feb): 334-8, 340-3.

    Impact of estrogen receptor beta on gene networks regulated by estrogen receptor alpha in breast cancer cells.
    Katzenellenbogen BS
    Endocrinology 147.10 (2006 Oct): 4831-42.

    17 beta-estradiol inhibits cardiac fibroblast growth through both subtypes of estrogen receptor.
    Ouchi Y
    Biochemical and biophysical research communications 311.2 (2003 Nov 14): 454-9.

    Estrogen receptor beta mediates the inhibitory effect of estradiol on vascular smooth muscle cell proliferation.
    Ouchi Y
    Cardiovascular research 59.3 (2003 Sep 1): 734-44.

    ERβ- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis.
    Bulun SE
    Molecular endocrinology (Baltimore, Md.) 28.8 (2014 Aug): 1304-15.

    Estrogen response elements alter coactivator recruitment through allosteric modulation of estrogen receptor beta conformation.
    Nardulli AM
    The Journal of biological chemistry 276.48 (2001 Nov 30): 45282-8.

    On the problem of oncogene of tumour viruses.
    Mekler LB
    Acta virologica 19.6 (1975 Nov): 501-8.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review