Cofilin (poly)

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$45.00
SKU: Cofilin (poly)-p
View product citations for antibody Cofilin (poly) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cofilin
Available to For-Profits: Yes
Antigen Species: Saccharomyces cerevisiae
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Cooper, J.A.
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Saccharomyces cerevisiae
Depositors Institution: Washington University, St. Louis
Antigen Molecular Weight: 15.9 kDa
Depositors Notes: Primary antibody dilution for immunostaining is 1:1000.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant His-Cof1 fusion protein expressed in E. coli
Gene: COF1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q03048 
Epitope Location or Sequence:
Entrez Gene ID: 850676 
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2889817 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RRID: AB_2889817
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cofilin (poly) was deposited to the DSHB by Cooper, J.A. (DSHB Hybridoma Product Cofilin (poly))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo.
    Cooper JA
    Journal of cell science 123.Pt 8 (2010 Apr 15): 1329-42.

    IF References

    Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo.
    Cooper JA
    Journal of cell science 123.Pt 8 (2010 Apr 15): 1329-42.

    All References

    Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo.
    Cooper JA
    Journal of cell science 123.Pt 8 (2010 Apr 15): 1329-42.

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