CrebA Rbt-PC
Catalog Fields
Antigen: Cyclic AMP response element-binding protein A; CrebA
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Andrew, D.J. / Hanlon, C.D.
Isotype: RIgG
Depositors Institution: Johns Hopkins University School of Medicine
Host Species: rabbit
Depositors Notes: Recommended dilution 1:50,000.
Positive Tested Species Reactivity: Drosophila
Gene: CrebA
Antigen Molecular Weight: 56.3 kDa
Alternate Gene Names: Bbbf2
Immunogen: Recombinant CrebA protein isolated from BL21(DE3) cell inclusion bodies
Clonality: Polyclonal
Uniprot ID: P29747
Epitope Mapped: No
Entrez Gene ID: 39682
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_10805295
Additional Information: Rrid: AB_10805295 FBgn0004396.
Recommended Applications: Chromatin Immunoprecipitation, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CrebA Rbt-PC was deposited to the DSHB by Andrew, D.J. / Hanlon, C.D. (DSHB Hybridoma Product CrebA Rbt-PC)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle.
Henderson KD
Development (Cambridge, England) 124.1 (1997 Jan): 181-93.