Catalog Fields
Antigen: Cyclic AMP response element-binding protein A; CrebA
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Andrew, D.J. / Hanlon, C.D.
Isotype: Rabbit IgG
Antigen Sequence:
Host Species: rabbit
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Recommended dilution 1:50,000.
Predicted Species Reactivity:
Gene: CrebA
Immunogen: Recombinant CrebA protein isolated from BL21(DE3) cell inclusion bodies
Alternate Gene Names: Bbbf2
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P29747
Immunogen Sequence: Full length protein
Entrez Gene ID: 39682
Antibody Registry ID: AB_10805295
Additional Information: Rrid: AB_10805295 FBgn0004396.
Recommended Applications: Chromatin Immunoprecipitation, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CrebA Rbt-PC was deposited to the DSHB by Andrew, D.J. / Hanlon, C.D. (DSHB Hybridoma Product CrebA Rbt-PC)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle.
Henderson KD
Development (Cambridge, England) 124.1 (1997 Jan): 181-93.