D5

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SKU: D5

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DSHB Data Sheet

Catalog Fields

Product Name/ID: D5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: LAMB2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528329 
Uniprot ID: E1BDK6 
RRID:  
Entrez Gene ID: 520167 
Clonality: Monoclonal
Immunogen: Adult bovine anterior lens capsule
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: fox NY
Epitope Mapped: Yes
Antigen Name: laminin, beta-2
Epitope Location or Sequence: C-terminal, domain I region
Alternate Antigen Name:
Deposit Date: 7/22/1992
Antigen Molecular Weight: Predicted: 192 kDa; Apparent: 190 kDa (reducing conditions)
Depositor: Sanes, J.R.
Antigen Sequence:
Depositor Institution: Harvard University
Antigen Species: Bovine
Depositor Notes: D5 blocks binding of neurons to s-laminin fusion protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Guinea Pig, Rabbit, Rat
Additional Information: The former name of laminin beta-2 was S-laminin. D5 doesn't cross-react with mouse antigen. C4, D5 and D7 recognize different epitopes.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D5 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

    IF References

    A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction.
    Sanes JR
    Nature 338.6212 (1989 Mar 16): 229-34.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Expression of s-laminin and laminin in the developing rat central nervous system.
    Sanes JR
    The Journal of comparative neurology 323.2 (1992 Sep 8): 238-51.

    A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin).
    Sanes JR
    Science (New York, N.Y.) 269.5222 (1995 Jul 21): 413-6.

    Characterization of bone marrow laminins and identification of alpha5-containing laminins as adhesive proteins for multipotent hematopoietic FDCP-Mix cells.
    Ekblom M
    Blood 93.8 (1999 Apr 15): 2533-42.

    Differential laminin isoform expression in the developing rat olfactory system.
    Schwarting GA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 187-96.

    Composition in situ and in vitro of vascular smooth muscle laminin in the rat.
    McGuffee LJ
    Cell and tissue research 281.1 (1995 Jul): 187-96.

    S-laminin and N-acetylgalactosamine located at the synaptic basal lamina of skeletal muscle are involved in synaptic recognition by growing neurites.
    Comella JX
    Journal of neurocytology 24.12 (1995 Dec): 903-15.

    Diabetes induces changes in glomerular development and laminin-beta 2 (s-laminin) expression.
    Abrahamson DR
    The American journal of pathology 151.4 (1997 Oct): 1131-40.

    A missense LAMB2 mutation causes congenital nephrotic syndrome by impairing laminin secretion.
    Miner JH
    Journal of the American Society of Nephrology : JASN 22.5 (2011 May): 849-58.

    WB References
    IP References

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    FB References

    A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction.
    Sanes JR
    Nature 338.6212 (1989 Mar 16): 229-34.

    S-laminin and N-acetylgalactosamine located at the synaptic basal lamina of skeletal muscle are involved in synaptic recognition by growing neurites.
    Comella JX
    Journal of neurocytology 24.12 (1995 Dec): 903-15.

    An LRE (leucine-arginine-glutamate)-dependent mechanism for adhesion of neurons to S-laminin.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 11.12 (1991 Dec): 3960-71.

    Epitope Map References
    All References

    A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction.
    Sanes JR
    Nature 338.6212 (1989 Mar 16): 229-34.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Expression of s-laminin and laminin in the developing rat central nervous system.
    Sanes JR
    The Journal of comparative neurology 323.2 (1992 Sep 8): 238-51.

    A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin).
    Sanes JR
    Science (New York, N.Y.) 269.5222 (1995 Jul 21): 413-6.

    Characterization of bone marrow laminins and identification of alpha5-containing laminins as adhesive proteins for multipotent hematopoietic FDCP-Mix cells.
    Ekblom M
    Blood 93.8 (1999 Apr 15): 2533-42.

    Differential laminin isoform expression in the developing rat olfactory system.
    Schwarting GA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 187-96.

    Composition in situ and in vitro of vascular smooth muscle laminin in the rat.
    McGuffee LJ
    Cell and tissue research 281.1 (1995 Jul): 187-96.

    S-laminin and N-acetylgalactosamine located at the synaptic basal lamina of skeletal muscle are involved in synaptic recognition by growing neurites.
    Comella JX
    Journal of neurocytology 24.12 (1995 Dec): 903-15.

    Diabetes induces changes in glomerular development and laminin-beta 2 (s-laminin) expression.
    Abrahamson DR
    The American journal of pathology 151.4 (1997 Oct): 1131-40.

    A missense LAMB2 mutation causes congenital nephrotic syndrome by impairing laminin secretion.
    Miner JH
    Journal of the American Society of Nephrology : JASN 22.5 (2011 May): 849-58.

    Purification and lectin-binding properties of s-laminin, a synaptic isoform of the laminin B1 chain.
    Ko J
    Journal of neurochemistry 59.1 (1992 Jul): 10-7.

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    An LRE (leucine-arginine-glutamate)-dependent mechanism for adhesion of neurons to S-laminin.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 11.12 (1991 Dec): 3960-71.

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

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