D5
Catalog Fields
Antigen: laminin, beta-2
Available to For-Profits: Yes
Antigen Species: Bovine
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Sanes, J.R.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Guinea Pig, Rabbit, Rat
Depositors Institution: Harvard University
Antigen Molecular Weight: Predicted: 192 kDa; Apparent: 190 kDa (reducing conditions)
Depositors Notes: D5 blocks binding of neurons to s-laminin fusion protein.
Immunogen: Adult bovine anterior lens capsule
Gene: LAMB2
Alternate Antibody Name:
Alternate Gene Names: LAMS
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: fox NY
Epitope Mapped: Yes
Uniprot ID: E1BDK6
Epitope Location or Sequence: C-terminal, domain I region
Entrez Gene ID: 520167
Immunogen Sequence: full length protein
Antibody Registry ID: AB_528329
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: The former name of laminin beta-2 was S-laminin. D5 doesn't cross-react with mouse antigen. C4, D5 and D7 recognize different epitopes.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D5 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
The basal lamina of the neuromuscular junction.
Chiu AY
Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.