D7

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$40.00
SKU: D7
View product citations for antibody D7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin, beta-2
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Sanes, J.R.
Isotype: MIgG1
Depositors Institution: Harvard University
Host Species: mouse
Gene: LAMB2
Positive Tested Species Reactivity: Canine, Guinea Pig, Rabbit, Rat
Alternate Gene Names: LAMS
Antigen Molecular Weight: Predicted: 192 kDa; Apparent: 190 kDa (reducing conditions)
Clonality: Monoclonal
Immunogen: Adult bovine anterior lens capsule
Epitope Mapped: Yes
Myeloma Strain: fox NY
Epitope Location or Sequence: C-terminal, domain I region
Uniprot ID: E1BDK6 
Immunogen Sequence: full length protein
Entrez Gene ID: 520167 
Additional Information: The former name of laminin beta-2 was S-laminin. D7 doesn't cross-react with mouse antigen. D7 doesn't appear to affect laminin beta-2 function. C4, D5 and D7 recognize different epitopes.
Antibody Registry ID: AB_528330 
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D7 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

    IF References
    WB References

    A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin)
    Sanes JR
    Science (New York, N.Y.) 269.5222 (1995 Jul 21): 413-6.

    Composition in situ and in vitro of vascular smooth muscle laminin in the rat.
    McGuffee LJ
    Cell and tissue research 281.1 (1995 Jul): 187-96.

    Purification and lectin-binding properties of s-laminin, a synaptic isoform of the laminin B1 chain.
    Ko J
    Journal of neurochemistry 59.1 (1992 Jul): 10-7.

    IP References

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    ELISA References
    Epitope Map References
    All References

    A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction.
    Sanes JR
    Nature 338.6212 (1989 Mar 16): 229-34.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Expression of s-laminin and laminin in the developing rat central nervous system.
    Sanes JR
    The Journal of comparative neurology 323.2 (1992 Sep 8): 238-51.

    A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin)
    Sanes JR
    Science (New York, N.Y.) 269.5222 (1995 Jul 21): 413-6.

    Composition in situ and in vitro of vascular smooth muscle laminin in the rat.
    McGuffee LJ
    Cell and tissue research 281.1 (1995 Jul): 187-96.

    S-laminin and N-acetylgalactosamine located at the synaptic basal lamina of skeletal muscle are involved in synaptic recognition by growing neurites.
    Comella JX
    Journal of neurocytology 24.12 (1995 Dec): 903-15.

    A missense LAMB2 mutation causes congenital nephrotic syndrome by impairing laminin secretion.
    Miner JH
    Journal of the American Society of Nephrology : JASN 22.5 (2011 May): 849-58.

    Purification and lectin-binding properties of s-laminin, a synaptic isoform of the laminin B1 chain.
    Ko J
    Journal of neurochemistry 59.1 (1992 Jul): 10-7.

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    Inhibition of endothelial cell adhesion and proliferation by extracellular matrix from vascular smooth muscle cells: role of type V collagen.
    Whitelock JM
    Atherosclerosis 141.1 (1998 Nov): 141-52.

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

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