Antigen: T-box transcription factor T/ Brachyury protein
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Alfandari, D.
Host Species: mouse
Depositors Institution: University of Massachusetts, Amherst
Positive Tested Species Reactivity: Xenopus
Predicted Species Reactivity:
Immunogen: Recombinant protein
Alternate Gene Names: X-bra, Xbra, Xbrachyury, bra, brachyury,
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P24781
Immunogen Sequence: Full Length
Entrez Gene ID: 493501
Antibody Registry ID: AB_2876386
Additional Information: Please reference NIH Grant R24OD21485 in all publications.
Recommended Applications: ELISA, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA12H2 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA12H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).