(0)No Reviews yet
$50.00 to $600.00

In Stock

Available: 189

DSHB Data Sheet

Catalog Fields

Product Name/ID: DA1D1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Sox9
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2876374 
Uniprot ID: Q6F2E7 
Entrez Gene ID: 549607 
Clonality: Monoclonal
Immunogen: Recombinant protein expressed in HEK293
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Transcription factor Sox-9
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/26/2020
Antigen Molecular Weight: 53.9 kDa
Depositor: Alfandari, D.
Antigen Sequence:
Depositor Institution: University of Massachusetts, Amherst
Antigen Species: Xenopus
Depositor Notes: This antigen is also known as Xt-sox9 or SRY-box 9.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: Please reference NIH Grant R24OD21485 in all publications.
Predicted Species Reactivity:  
Human Protein Atlas: http://AB_2876374 
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA1D1 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA1D1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    . (): .

    All References

    . (): .

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review