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SKU: DA4Ascl1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: DA4Ascl1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ascl1
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: Q06234 
Entrez Gene ID: 378517 
Clonality: Monoclonal
Immunogen: Recombinant protein expressed in Hek293T
Immunogen Sequence: Full Length
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Achaete-scute homolog 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/3/2021
Antigen Molecular Weight: 22.3 kDa
Depositor: Alfandari, D.
Antigen Sequence:
Depositor Institution: University of Massachusetts, Amherst
Antigen Species: Xenopus
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: Please reference NIH Grant R24OD21485 in alll publications.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA4Ascl1 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA4Ascl1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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