DAG-6F4

(0) No Reviews yet
$40.00
SKU: DAG-6F4
View product citations for antibody DAG-6F4 on CiteAb

In Stock

Available: 190

DSHB Data Sheet

Catalog Fields

Antigen: Dystroglycan, alpha-
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes:
Antigen Molecular Weight: 156 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DAG1
Immunogen: Protein domain of alpha-dystroglycan linked to thioredoxin
Alternate Gene Names:
Alternate Antibody Name: 6F4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa-PNQRPEL by phage display
Uniprot ID: Q14118 
Immunogen Sequence: a.a. 312-653
Entrez Gene ID: 1605 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DAG-6F4 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product DAG-6F4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

Ratings & Reviews

No reviews available

Be the first to Write a Review