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View product citations for antibody DAG-6F4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Dystroglycan, alpha-
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2a
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Gene: DAG1
Positive Tested Species Reactivity: Human, Mouse
Clonality: Monoclonal
Antigen Molecular Weight: 156 kDa
Epitope Mapped: Yes
Immunogen: Protein domain of alpha-dystroglycan linked to thioredoxin
Epitope Location or Sequence: aa-PNQRPEL by phage display
Alternate Antibody Name: 6F4
Immunogen Sequence: a.a. 312-653
Myeloma Strain: SP2/0
Uniprot ID: Q14118 
Entrez Gene ID: 1605 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DAG-6F4 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product DAG-6F4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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