DPDF-P (nb33)

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$40.00
SKU: DPDF-P (nb33)
View product citations for antibody DPDF-P (nb33) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Pigment dispersing factor (PDF) precursor-related peptide
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg, Germany
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes cleaved PDF protein precursor-related peptide. nb33 selectively stains large ventral lateral neurons (1LNv) and small ventral lateral neurons (sLNv) .
Antigen Molecular Weight: 11.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PDF
Immunogen: Brain homogenate of transgenic flies expressing a.a. 1-82 of PDF precursor in PDF01 null background.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: O96690 
Immunogen Sequence: a.a. 1-82
Entrez Gene ID: 43193 
Additional Characterization:  
Antibody Registry ID: AB_10805428 
Additional Information: FBgn0023178. RRID:AB_10805428
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DPDF-P (nb33) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DPDF-P (nb33))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    WB References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    IHC References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    All References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

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