DPDF-P (nb33)

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$45.00 to $425.00
SKU: DPDF-P (nb33)
View product citations for antibody DPDF-P (nb33) on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Pigment dispersing factor (PDF) precursor-related peptide
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Buchner, E. / Hofbauer, A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of Wuerzburg, Germany
Antigen Molecular Weight: 11.5 kDa
Depositors Notes: This antibody recognizes cleaved PDF protein precursor-related peptide. nb33 selectively stains large ventral lateral neurons (1LNv) and small ventral lateral neurons (sLNv) .
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Brain homogenate of transgenic flies expressing a.a. 1-82 of PDF precursor in PDF01 null background.
Gene: PDF
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X.63-Ag8.653
Epitope Mapped: No
Uniprot ID: O96690 
Epitope Location or Sequence:
Entrez Gene ID: 43193 
Immunogen Sequence: a.a. 1-82
Antibody Registry ID: AB_10805428 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information: FBgn0023178. RRID:AB_10805428
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DPDF-P (nb33) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DPDF-P (nb33))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    WB References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    IHC References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    All References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

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