Antigen: Luciferase Luciferin 4-monooxygenase
Antigen Species: Photinus pyralis
Depositors Institution: University of Iowa
Depositors Notes: This antigen is also known as luciferin 4-monooxygenase.
Host Species: mouse
Positive Tested Species Reactivity: Photinus pyralis. All species as fusion proteins.
Immunogen Sequence: Full length protein
Antigen Molecular Weight: 60.7 kDa
Additional Information: RRID:AB_2722107
Immunogen: Recombinant luciferase expressed in E. coli
Myeloma Strain: Sp2/0-Ag14
Uniprot ID: P08659
Antibody Registry ID: AB_2722107
Recommended Applications: FFPE, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-LUC-2 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-LUC-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).