DSHB-MBP-2A1

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$40.00
SKU: DSHB-MBP-2A1-s
View product citations for antibody DSHB-MBP-2A1 on CiteAb

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Available: 69

DSHB Data Sheet

Catalog Fields

Antigen: Maltose binding protein
Hybridoma Cells Available: No
Antigen Species: E. coli
Depositor: DSHB
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: All species for fusion proteins, E. coli
Depositors Notes: This antigen tag is also known as extracellular solute-binding protein family 1 or MalE protein. MBP was expressed in E. coli using the pDB.His.MBP vector.
Antigen Molecular Weight: 42.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: malE
Immunogen: Recombinant Protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: C3SHQ8 
Immunogen Sequence: Full length protein
Entrez Gene ID: 948538 
Additional Characterization:  
Antibody Registry ID: AB_2617428 
Additional Information: RRID:AB_2617428
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-MBP-2A1 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-MBP-2A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    WB References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    IP References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    ELISA References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    All References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

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