DSHB-MBP-2A1

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$50.00
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SKU: DSHB-MBP-2A1-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: DSHB-MBP-2A1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: malE
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2617428 
Uniprot ID: C3SHQ8 
RRID:  
Entrez Gene ID: 948538 
Clonality: Monoclonal
Immunogen: Recombinant Protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: Maltose binding protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/4/2015
Antigen Molecular Weight: 42.5 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: E. coli
Depositor Notes: This antigen tag is also known as extracellular solute-binding protein family 1 or MalE protein. MBP was expressed in E. coli using the pDB.His.MBP vector.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: All species for fusion proteins, E. coli
Additional Information: RRID:AB_2617428
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-MBP-2A1 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-MBP-2A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    WB References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    IP References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    ELISA References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

    All References

    Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
    Soll DR
    Monoclonal antibodies in immunodiagnosis and immunotherapy 35.2 (2016 Apr): 104-8.

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