Dm 4B8/F12
Catalog Fields
Clone ID/Product Name: Dm 4B8/F12
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: cheerio
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_3094862
Uniprot ID: Q9VEN1
Entrez Gene ID: 42066
Clonality: Monoclonal
Immunogen: Larval l(3)mbn-1 hemocytes
Clone:
Immunogen Sequence: Full-length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Filamin-240
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/22/2024
Antigen Molecular Weight: 240 kDa
Depositor: Ando, Istvan / Kurucz, Eva
Antigen Sequence:
Depositor Institution: HUN-REN Biological Research Centre, Szeged
Antigen Species: Drosophila melanogaster
Depositor Notes: Lamellocyte, sub-population/ L5 CD antigen
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The cells were fixed with acetone for 6 minutes, air dried and rehydrated with PBS solution for 10 minutes and blocked with PBS containing 0.1% BSA.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dm 4B8/F12 was deposited to the DSHB by Ando, Istvan / Kurucz, Eva (DSHB Hybridoma Product Dm 4B8/F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ‘l for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ‘g/ml. For western blots, the recommended concentration range is 0.2-0.5 ‘g/ml.
1 Reference
Initial Publication
Definition of Drosophila hemocyte subsets by cell-type specific antigens.
Andó I
Acta biologica Hungarica 58 Suppl. (2007): 95-111.