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SKU: EH17a

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Available: 43

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: EH17a
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_528080 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: calcium-dependent phospholipid-binding proteins
Immunogen Sequence:
Myeloma Strain: P3X653-Ag8
Epitope Mapped: No
Antigen Name: annexin I
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/8/1994
Antigen Molecular Weight: 39 kDa
Depositor: Ernst, J.D.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: human
Depositor Notes: Functional effects notes: does not block calcium or phospholipid binding; does not block vesicle aggregation
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EH17a was deposited to the DSHB by Ernst, J.D. (DSHB Hybridoma Product EH17a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • All References
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