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SKU: ENCITp300-1-s
View product citations for antibody ENCITp300-1 on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Product Name/ID: ENCITp300-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: EP300
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619604 
Uniprot ID: Q7Z6C1 
Entrez Gene ID: 2033 
Clonality: Monoclonal
Immunogen Sequence:
Myeloma Strain: HL-1 myeloma
Epitope Mapped: Yes
Antigen Name: EP300
Epitope Location or Sequence: aa 1526-1545
Alternate Antigen Name:
Deposit Date: 3/1/2014
Antigen Molecular Weight: 228.2 kDa
Depositor: Wold, B. / Vielmetter, J.
Antigen Sequence: aa 1526-1545
Depositor Institution: California Institute of Technology
Antigen Species: Human
Depositor Notes: PFA or Bouins; AR with citrate buffer
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse
Additional Information: RRID:AB_2619604
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ENCITp300-1 was deposited to the DSHB by Wold, B. / Vielmetter, J. (DSHB Hybridoma Product ENCITp300-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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