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SKU: ENCITp300-1-s
View product citations for antibody ENCITp300-1 on CiteAb

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Available: 68

DSHB Data Sheet

Catalog Fields

Antigen: EP300
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Wold, B. / Vielmetter, J.
Isotype: MIgG1
Antigen Sequence: aa 1526-1545
Host Species: mouse
Depositors Institution: Cal Tech
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: PFA or Bouins; AR with citrate buffer
Antigen Molecular Weight: 228.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: EP300
Alternate Gene Names: RP1-85F18.1, KAT3B, RSTS2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1 myeloma
Epitope Location or Sequence: aa 1526-1545
Uniprot ID: Q7Z6C1 
Immunogen Sequence:
Entrez Gene ID: 2033 
Additional Characterization:  
Antibody Registry ID: AB_2619604 
Additional Information: RRID:AB_2619604
Recommended Applications: Chromatin Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ENCITp300-1 was deposited to the DSHB by Wold, B. / Vielmetter, J. (DSHB Hybridoma Product ENCITp300-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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