ENCITp300-1

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$40.00
SKU: ENCITp300-1-s
View product citations for antibody ENCITp300-1 on CiteAb

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Available: 159

DSHB Data Sheet

Catalog Fields

Antigen: EP300
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Wold, B. / Vielmetter, J.
Isotype: MIgG1
Antigen Sequence: aa 1526-1545
Host Species: mouse
Depositors Institution: Cal Tech
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: PFA or Bouins; AR with citrate buffer
Antigen Molecular Weight: 228.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: EP300
Immunogen:
Alternate Gene Names: RP1-85F18.1, KAT3B, RSTS2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1 myeloma
Epitope Location or Sequence: aa 1526-1545
Uniprot ID: Q7Z6C1 
Immunogen Sequence:
Entrez Gene ID: 2033 
Additional Characterization:
Antibody Registry ID: AB_2619604 
Additional Information: RRID:AB_2619604
Recommended Applications: Chromatin Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ENCITp300-1 was deposited to the DSHB by Wold, B. / Vielmetter, J. (DSHB Hybridoma Product ENCITp300-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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