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SKU: ENS-1-s
View product citations for antibody ENS-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ENS-1; Embryonic Normal Stem Cell
Hybridoma Cells Available: No
Antigen Species: Chicken
Depositor: Mey, A.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universitat Lyon
Positive Tested Species Reactivity: Chicken
Depositors Notes: The ENS-1 protein is distributed in the membrane, in the cytoplasm and in the nucleus of chicken ES cells. Permeabilization with 0.1% Triton X-100 is required for a full detection of the protein by this antibody
Antigen Molecular Weight: 54.7
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ENS-1
Immunogen: Recombinant protein
Alternate Gene Names: ERNI
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2OAg/14
Epitope Location or Sequence:
Uniprot ID: Q98TV2 
Immunogen Sequence:
Entrez Gene ID: 769415 
Additional Characterization:  
Antibody Registry ID: AB_2618116 
Additional Information: Not suitable for Western blot. RRID:AB_2618116
Recommended Applications: ELISA, FFPE, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ENS-1 was deposited to the DSHB by Mey, A. (DSHB Hybridoma Product ENS-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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  • Initial Publication
    IF References
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