ES1

(0) No Reviews yet
$40.00
SKU: ES1
View product citations for antibody ES1 on CiteAb

In Stock

Available: 8

DSHB Data Sheet

Catalog Fields

Antigen: E-selectin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: McEver, R.P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oklahoma Medical Research Foundation
Positive Tested Species Reactivity: Human
Depositors Notes: ES1 may be used for western blotting under non-reducing conditions.
Antigen Molecular Weight: 66.65 kDa/110 kDa glycosylated
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: SELE
Immunogen: soluble E-selectin
Alternate Gene Names: ELAM1. CD62E
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3-X63-Ag8-653
Epitope Location or Sequence:
Uniprot ID: P16581 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 6401 
Additional Characterization:
Antibody Registry ID:
Additional Information:
Recommended Applications: Function Blocking, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ES1 was deposited to the DSHB by McEver, R.P. (DSHB Hybridoma Product ES1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • FB References
  • All References
  • Initial Publication

    Neutrophils use both shared and distinct mechanisms to adhere to selectins under static and flow conditions.
    McEver RP
    The Journal of clinical investigation 96.4 (1995 Oct): 1887-96.

    FB References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review