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View product citations for antibody ES1 on CiteAb

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Available: 8

DSHB Data Sheet

Catalog Fields

Antigen: E-selectin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: McEver, R.P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oklahoma Medical Research Foundation
Positive Tested Species Reactivity: Human
Depositors Notes: ES1 may be used for western blotting under non-reducing conditions.
Antigen Molecular Weight: 66.65 kDa/110 kDa glycosylated
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SELE
Immunogen: soluble E-selectin
Alternate Gene Names: ELAM1. CD62E
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-X63-Ag8-653
Epitope Location or Sequence:
Uniprot ID: P16581 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 6401 
Additional Characterization:  
Antibody Registry ID: AB_2889816 
Additional Information: RRID: AB_2889816
Recommended Applications: Function Blocking, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ES1 was deposited to the DSHB by McEver, R.P. (DSHB Hybridoma Product ES1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • FB References
  • All References
  • Initial Publication

    Neutrophils use both shared and distinct mechanisms to adhere to selectins under static and flow conditions.
    McEver RP
    The Journal of clinical investigation 96.4 (1995 Oct): 1887-96.

    FB References
    All References

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