Eralpha BZ1

(0) No Reviews yet
$45.00 to $425.00
SKU: Eralpha BZ1
View product citations for antibody Eralpha BZ1 on CiteAb

In Stock

Available: 89

DSHB Data Sheet

Catalog Fields

Antigen: Estrogen receptor, alpha
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Nardulli, A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois, Urbana-Champaign
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as ER-alpha and the estradiol receptor. In gel mobilty shift assay, the BZ1 antibody binds to DNA-bound purified estrogen receptor.
Antigen Molecular Weight: 66 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ESR1
Immunogen: Recombinant protein domain
Alternate Gene Names: ESR, NR3A1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: P03372 
Immunogen Sequence: Lligand binding domain a.a. 304-554
Entrez Gene ID: 2099 
Additional Characterization:  
Antibody Registry ID: B_10805283 
Additional Information: RRID:AB_10805283
Recommended Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Eralpha BZ1 was deposited to the DSHB by Nardulli, A. (DSHB Hybridoma Product Eralpha BZ1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • ChIP References
  • All References
  • Initial Publication
    WB References
    IP References
    ChIP References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review