Eralpha BZ1

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SKU: Eralpha BZ1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Eralpha BZ1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ESR1
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: B_10805283 
Uniprot ID: P03372 
RRID:  
Entrez Gene ID: 2099 
Clonality: Monoclonal
Immunogen: Recombinant protein domain
Clone:
Immunogen Sequence: Lligand binding domain a.a. 304-554
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: Estrogen receptor, alpha
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/1/2011
Antigen Molecular Weight: 66 kDa
Depositor: Nardulli, A.
Antigen Sequence:
Depositor Institution: University of Illinois, Urbana-Champaign
Antigen Species: Human
Depositor Notes: This antigen is also known as ER-alpha and the estradiol receptor. In gel mobilty shift assay, the BZ1 antibody binds to DNA-bound purified estrogen receptor.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_10805283
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Eralpha BZ1 was deposited to the DSHB by Nardulli, A. (DSHB Hybridoma Product Eralpha BZ1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • ChIP References
  • All References
  • Initial Publication
    WB References
    IP References
    ChIP References
    All References

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