Even-skipped
Catalog Fields
Clone ID/Product Name: Even-skipped
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: eve
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_2892600
Uniprot ID: P06602
Entrez Gene ID: 36039
Clonality: Polyclonal
Immunogen: T7 tag fusion protein expressed in E. coli (from pET-3 vector)
Clone:
Immunogen Sequence: a.a. 1-376
Myeloma Strain: NA
Epitope Mapped: No
Antigen Name: Segmentation protein even-skipped
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/30/2021
Antigen Molecular Weight: 39.9 kDa
Depositor: Frasch, M.
Antigen Sequence:
Depositor Institution: Universitaet Erlangen- Nuernberg
Antigen Species: Drosophila
Depositor Notes: Recommended dilution 1:3000
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: FBgn0000606
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Even-skipped was deposited to the DSHB by Frasch, M. (DSHB Hybridoma Product Even-skipped)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
Characterization and localization of the even-skipped protein of Drosophila.
Levine M
The EMBO journal 6.3 (1987 Mar): 749-59.