Even-skipped

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$45.00
SKU: Even-skipped-p
View product citations for antibody Even-skipped on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Segmentation protein even-skipped
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Frasch, M.
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Universitaet Erlangen- Nuernberg
Antigen Molecular Weight: 39.9 kDa
Depositors Notes: Recommended dilution 1:3000
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: T7 tag fusion protein expressed in E. coli (from pET-3 vector)
Gene: eve
Alternate Antibody Name:
Alternate Gene Names: CG2328
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain: NA
Epitope Mapped: No
Uniprot ID: P06602 
Epitope Location or Sequence:
Entrez Gene ID: 36039 
Immunogen Sequence: a.a. 1-376
Antibody Registry ID: AB_2892600 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: FBgn0000606
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Even-skipped was deposited to the DSHB by Frasch, M. (DSHB Hybridoma Product Even-skipped)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References

    Characterization and localization of the even-skipped protein of Drosophila.
    Levine M
    The EMBO journal 6.3 (1987 Mar): 749-59.

    Complementary patterns of even-skipped and fushi tarazu expression involve their differential regulation by a common set of segmentation genes in Drosophila.
    Levine M
    Genes & development 1.9 (1987 Nov): 981-95.

    tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
    Frasch M
    Genes & development 7.7B (1993 Jul): 1325-40.

    The Drosophila homeobox genes zfh-1 and even-skipped are required for cardiac-specific differentiation of a numb-dependent lineage decision.
    Bodmer R
    Development (Cambridge, England) 126.14 (1999 Jun): 3241-51.

    Molecular integration of inductive and mesoderm-intrinsic inputs governs even-skipped enhancer activity in a subset of pericardial and dorsal muscle progenitors.
    Frasch M
    Developmental biology 238.1 (2001 Oct 1): 13-26.

    Analysis of an even-skipped rescue transgene reveals both composite and discrete neuronal and early blastoderm enhancers, and multi-stripe positioning by gap gene repressor gradients.
    Jaynes JB
    Development (Cambridge, England) 126.11 (1999 Jun): 2527-38.

    Embryonic even skipped-dependent muscle and heart cell fates are required for normal adult activity, heart function, and lifespan.
    Jaynes JB
    Circulation research 97.11 (2005 Nov 25): 1108-14.

    Even-Skipped(+) Interneurons Are Core Components of a Sensorimotor Circuit that Maintains Left-Right Symmetric Muscle Contraction Amplitude.
    Doe CQ
    Neuron 88.2 (2015 Oct 21): 314-29.

    Drosophila MEF2, a transcription factor that is essential for myogenesis.
    Nguyen HT
    Genes & development 9.6 (1995 Mar 15): 730-41.

    All References

    Characterization and localization of the even-skipped protein of Drosophila.
    Levine M
    The EMBO journal 6.3 (1987 Mar): 749-59.

    Complementary patterns of even-skipped and fushi tarazu expression involve their differential regulation by a common set of segmentation genes in Drosophila.
    Levine M
    Genes & development 1.9 (1987 Nov): 981-95.

    tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
    Frasch M
    Genes & development 7.7B (1993 Jul): 1325-40.

    The Drosophila homeobox genes zfh-1 and even-skipped are required for cardiac-specific differentiation of a numb-dependent lineage decision.
    Bodmer R
    Development (Cambridge, England) 126.14 (1999 Jun): 3241-51.

    Molecular integration of inductive and mesoderm-intrinsic inputs governs even-skipped enhancer activity in a subset of pericardial and dorsal muscle progenitors.
    Frasch M
    Developmental biology 238.1 (2001 Oct 1): 13-26.

    Analysis of an even-skipped rescue transgene reveals both composite and discrete neuronal and early blastoderm enhancers, and multi-stripe positioning by gap gene repressor gradients.
    Jaynes JB
    Development (Cambridge, England) 126.11 (1999 Jun): 2527-38.

    Embryonic even skipped-dependent muscle and heart cell fates are required for normal adult activity, heart function, and lifespan.
    Jaynes JB
    Circulation research 97.11 (2005 Nov 25): 1108-14.

    Even-Skipped(+) Interneurons Are Core Components of a Sensorimotor Circuit that Maintains Left-Right Symmetric Muscle Contraction Amplitude.
    Doe CQ
    Neuron 88.2 (2015 Oct 21): 314-29.

    Drosophila MEF2, a transcription factor that is essential for myogenesis.
    Nguyen HT
    Genes & development 9.6 (1995 Mar 15): 730-41.

    The embryonic central nervous system lineages of Drosophila melanogaster. I. Neuroblast lineages derived from the ventral half of the neuroectoderm.
    Technau GM
    Developmental biology 179.1 (1996 Oct 10): 41-64.

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