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View product citations for antibody F1C3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Fibrillar collagens; alpha 1(1), alpha 1 (III), alpha 2(V) and alpha 4 (V)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Faissner, Andreas
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Ruhr University, Bochum, Germany
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: 137.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Col1a1
Immunogen: irradiated A7 rat astrocyte cell line
Alternate Gene Names: COLIA1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence: GPPGPVG (aa 885-891, Type I alpha 1)
Uniprot ID: P02454 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 29393 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F1C3 was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product F1C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    IF References
    WB References
    IHC References
    IP References

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    ELISA References

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    Epitope Map References
    All References

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