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DSHB Data Sheet

Catalog Fields

Product Name/ID: F1C3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Col1a1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: P02454 
Entrez Gene ID: 29393 
Clonality: Monoclonal
Immunogen: irradiated A7 rat astrocyte cell line
Immunogen Sequence: Full length sequence
Myeloma Strain: X63Ag8.653
Epitope Mapped: Yes
Antigen Name: Fibrillar collagens; alpha 1(1), alpha 1 (III), alpha 2(V) and alpha 4 (V)
Epitope Location or Sequence: GPPGPVG (aa 885-891, Type I alpha 1)
Alternate Antigen Name:
Deposit Date: 11/6/2020
Antigen Molecular Weight: 137.9 kDa
Depositor: Faissner, Andreas
Antigen Sequence:
Depositor Institution: Ruhr University, Bochum, Germany
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F1C3 was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product F1C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    IF References
    WB References
    IHC References
    IP References

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    ELISA References

    Astrocytes in culture express fibrillar collagen.
    Faissner A
    Glia 41.4 (2003 Mar): 382-92.

    Epitope Map References
    All References

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