FE-C6
Catalog Fields
Antigen: mesoderm, early, rodent
Hybridoma Cells Available: No
Antigen Species: rat
Depositor: Fenderson, B. / Eddy, M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Jefferson Medical College
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: carbohydrate epitope carried on glycolipids and glycoproteins (e.g., desialylated human alpha 1-acid glycoprotein): Gal beta 1-4 G1cNAc beta 1-6 R. Cell binding note: cell surface binding to rat and mouse testicular cells, and early mesoderm (requires neuraminidase treatment).
Predicted Species Reactivity:
Gene:
Immunogen: rat testicular cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Antibody Registry ID: AB_2211970
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FE-C6 was deposited to the DSHB by Fenderson, B. / Eddy, M. (DSHB Hybridoma Product FE-C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis.
Eddy EM
Developmental biology 100.2 (1983 Dec): 318-27.