FE-C6
Catalog Fields
Clone ID/Product Name: FE-C6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_2211970
Clonality: Monoclonal
Immunogen: rat testicular cells
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: mesoderm, early, rodent
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/12/1990
Antigen Molecular Weight:
Depositor: Fenderson, B. / Eddy, M.
Antigen Sequence:
Depositor Institution: Jefferson Medical College
Antigen Species: rat
Depositor Notes: carbohydrate epitope carried on glycolipids and glycoproteins (e.g., desialylated human alpha 1-acid glycoprotein): Gal beta 1-4 G1cNAc beta 1-6 R. Cell binding note: cell surface binding to rat and mouse testicular cells, and early mesoderm (requires neuraminidase treatment).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FE-C6 was deposited to the DSHB by Fenderson, B. / Eddy, M. (DSHB Hybridoma Product FE-C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis.
Eddy EM
Developmental biology 100.2 (1983 Dec): 318-27.