Fl(2)d 9G2

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SKU: Fl(2)d 9G2
View product citations for antibody Fl(2)d 9G2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Pre-mRNA-splicing regulator female-lethal(2)D
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgA, kappa light chain
Depositors Institution: Princeton University
Host Species: mouse
Depositors Notes: This antibody recognizes the Drosophila homolog of Wilms'-Tumor-1-Associated Protein.
Positive Tested Species Reactivity: Drosophila
Gene: fl(2)d
Antigen Molecular Weight: 59. 2 kDa
Alternate Gene Names: Dmel_CG6315
Immunogen: Recombinant (baculovirus) protein
Clonality: Monoclonal
Myeloma Strain: SP-2
Epitope Mapped: No
Uniprot ID: Q9Y091 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 36527 
Additional Information: RRID:AB_10567131 FBgn0000662.
Antibody Registry ID: AB_10567131 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Fl(2)d 9G2 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product Fl(2)d 9G2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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