GABRB2-R13

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$45.00
SKU: GABRB2-R13-p
View product citations for antibody GABRB2-R13 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Gamma-aminobutyric acid receptor subunit beta-2
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: De Blas, Angel L.
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Rat
Depositors Institution: University of Connecticut
Antigen Molecular Weight: 54.6 kDa
Depositors Notes: The synthetic peptide immunogen was coupled to a carrier protein by a C-end, which was added if not present in the peptide. IP, IB (1:100)
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant protein domain
Gene: Gabrb2
Alternate Antibody Name:
Alternate Gene Names: Gabrb-2
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: P63138 
Epitope Location or Sequence: amino acids 303-427 of mature protein
Entrez Gene ID: 25451 
Immunogen Sequence: Large Intracellular loop fusion protein
Antibody Registry ID: AB_2877086 
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Western Blot
Additional Information: Affinity-purified antibody also validated for IHC and IF.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GABRB2-R13 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product GABRB2-R13)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    WB References

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    Brain GABAA receptors studied with subunit-specific antibodies.
    De Blas AL
    Molecular neurobiology 12.1 (1996 Feb): 55-71.

    Synaptic and extrasynaptic GABAA receptor and gephyrin clusters.
    De Blas AL
    Progress in brain research 136. (2002): 157-80.

    Coexistence of two beta subunit isoforms in the same gamma-aminobutyric acid type A receptor.
    De Blas AL
    The Journal of biological chemistry 272.26 (1997 Jun 27): 16564-9.

    Epitope Map References

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    All References

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    Brain GABAA receptors studied with subunit-specific antibodies.
    De Blas AL
    Molecular neurobiology 12.1 (1996 Feb): 55-71.

    Synaptic and extrasynaptic GABAA receptor and gephyrin clusters.
    De Blas AL
    Progress in brain research 136. (2002): 157-80.

    Coexistence of two beta subunit isoforms in the same gamma-aminobutyric acid type A receptor.
    De Blas AL
    The Journal of biological chemistry 272.26 (1997 Jun 27): 16564-9.

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