GD1a-2a

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$40.00
SKU: GD1a-2a-s
View product citations for antibody GD1a-2a on CiteAb

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Available: 84

DSHB Data Sheet

Catalog Fields

Antigen: Ganglioside GD1a
Hybridoma Cells Available:
Antigen Species: Bovine
Depositor: Schnaar, R.L.
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Campylobacter, Human, Mouse, Rat, Vertebrates
Depositors Notes: GD1a-2a recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Antigen Molecular Weight: 1.84 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: KLH-conjugated bovine brain GD1a
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x653
Epitope Location or Sequence: GD1a
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2619568 
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. GD1a-2a binds better to GT1aalpha [PMIDs: 11878808 12390975]. Western blot references are for TLC immunooverlay. The isotype of this antibody was originally described as MIgG2a.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GD1a-2a was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GD1a-2a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    IF References
    WB References
    IHC References
    ELISA References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Structural requirements of anti-GD1a antibodies determine their target specificity.
    Sheikh KA
    Brain : a journal of neurology 131.Pt 7 (2008 Jul): 1926-39.

    Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids.
    Schnaar RL
    Neurochemical research 27.7-8 (2002 Aug): 847-55.

    FB References

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

    Epitope Map References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    All References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation.
    Schnaar RL
    Biochimica et biophysica acta 1686.3 (2005 Jan 5): 200-8.

    Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections.
    Lauc G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.8 (2007 Aug): 805-12.

    Differential distribution of major brain gangliosides in the adult mouse central nervous system.
    Heffer M
    PloS one 8.9 (2013): e75720.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 127.Pt 5 (2004 May): 1085-100.

    Structural requirements of anti-GD1a antibodies determine their target specificity.
    Sheikh KA
    Brain : a journal of neurology 131.Pt 7 (2008 Jul): 1926-39.

    Molecular mimicry in Campylobacter jejuni: role of the lipo-oligosaccharide core oligosaccharide in inducing anti-ganglioside antibodies.
    Fry BN
    FEMS immunology and medical microbiology 50.1 (2007 Jun): 27-36.

    Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids.
    Schnaar RL
    Neurochemical research 27.7-8 (2002 Aug): 847-55.

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

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