GD1a-2b

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$40.00
SKU: GD1a-2b-s
View product citations for antibody GD1a-2b on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Ganglioside GD1a
Hybridoma Cells Available: No
Antigen Species: Bovine
Depositor: Schnaar, R.L.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Depositors Notes: GD1a-2b recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Antigen Molecular Weight: 1.84 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: KLH-conjugated bovine brain GD1a
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x653
Epitope Location or Sequence: GD1a
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619569 
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. GD1a-2b cross-react with GT1aalpha [PMIDs: 11878808 12390975]. Western blot references include references for TLC immunooverlay.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GD1a-2b was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GD1a-2b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    IF References

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.



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    WB References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    Structural requirements of anti-GD1a antibodies determine their target specificity.
    Sheikh KA
    Brain : a journal of neurology 131.Pt 7 (2008 Jul): 1926-39.

    IHC References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation.
    Schnaar RL
    Biochimica et biophysica acta 1686.3 (2005 Jan 5): 200-8.

    Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections.
    Lauc G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.8 (2007 Aug): 805-12.

    Differential distribution of major brain gangliosides in the adult mouse central nervous system.
    Heffer M
    PloS one 8.9 (2013): e75720.

    ELISA References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Structural requirements of anti-GD1a antibodies determine their target specificity.
    Sheikh KA
    Brain : a journal of neurology 131.Pt 7 (2008 Jul): 1926-39.

    Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids.
    Schnaar RL
    Neurochemical research 27.7-8 (2002 Aug): 847-55.

    Epitope Map References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    All References

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