GD1b-1

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$40.00
SKU: GD1b-1-s
View product citations for antibody GD1b-1 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Ganglioside GD1b
Hybridoma Cells Available: No
Antigen Species: Bovine
Depositor: Schnaar, R.L.
Isotype: MIgG1
Depositors Institution: Johns Hopkins School of Medicine
Host Species: mouse
Depositors Notes: GD1b-1recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Clonality: Monoclonal
Antigen Molecular Weight: 1.84 kDa
Epitope Mapped: Yes
Immunogen: KLH-conjugated bovine brain GD1b
Epitope Location or Sequence: GD1b
Myeloma Strain: P3x653
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Antibody Registry ID: AB_2619570 
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GD1b-1 was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GD1b-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    IF References
    WB References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    IHC References
    ELISA References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    FB References

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

    Epitope Map References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    All References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Differential distribution of major brain gangliosides in the adult mouse central nervous system.
    Heffer M
    PloS one 8.9 (2013): e75720.

    Biosynthesis of the major brain gangliosides GD1a and GT1b.
    Schnaar RL
    Glycobiology 22.10 (2012 Oct): 1289-301.

    Distribution of mono-, di- and trisialo gangliosides in the brain of Actinopterygian fishes.
    Heffer M
    Biochimica et biophysica acta 1820.9 (2012 Sep): 1437-43.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

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