Catalog Fields
Antigen: Ganglioside GD1b
Available to For-Profits: Yes
Antigen Species: Bovine
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: Schnaar, R.L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Depositors Institution: Johns Hopkins School of Medicine
Antigen Molecular Weight: 1.84 kDa
Depositors Notes: GD1b-1recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Immunogen: KLH-conjugated bovine brain GD1b
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x653
Epitope Mapped: Yes
Antibody Registry ID: AB_2619570
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GD1b-1 was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GD1b-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
6 References
Initial Publication
Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
Shiekh KA
Analytical biochemistry 302.2 (2002 Mar 15): 276-84.