GEM7E(8D8)

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$40.00
SKU: GEM7E(8D8)
View product citations for antibody GEM7E(8D8) on CiteAb

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Available: 7

DSHB Data Sheet

Catalog Fields

Antigen: Gem-associated protein 7
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody is not validated for pig, fish or mouse.
Antigen Molecular Weight: 14.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GEMIN7
Immunogen: Gemin7 recombinant protein
Alternate Gene Names: FLJ13956
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9H840 
Immunogen Sequence: Full length protein
Entrez Gene ID: 79760 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID: AB_2753295 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GEM7E(8D8) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product GEM7E(8D8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Absence of gemin5 from SMN complexes in nuclear Cajal bodies.
    Morris GE
    BMC cell biology 8. (2007 Jul 18): 28.

    IF References

    Absence of gemin5 from SMN complexes in nuclear Cajal bodies.
    Morris GE
    BMC cell biology 8. (2007 Jul 18): 28.

    WB References

    Absence of gemin5 from SMN complexes in nuclear Cajal bodies.
    Morris GE
    BMC cell biology 8. (2007 Jul 18): 28.

    All References

    Absence of gemin5 from SMN complexes in nuclear Cajal bodies.
    Morris GE
    BMC cell biology 8. (2007 Jul 18): 28.

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