GM1-2b

(0)No Reviews yet
$45.00
SKU: GM1-2b-s
View product citations for antibody GM1-2b on CiteAb

In Stock

Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: Ganglioside GM1
Available to For-Profits: Yes
Antigen Species: Bovine
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2b
Depositor: Schnaar, R.L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Depositors Institution: Johns Hopkins School of Medicine
Antigen Molecular Weight: 1.55 kDa
Depositors Notes: GM1-2b recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: KLH-conjugated bovine brain GM1
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x653
Epitope Mapped: Yes
Uniprot ID:  
Epitope Location or Sequence: GM1
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2619572 
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GM1-2b was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GM1-2b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    IF References
    WB References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    IHC References
    ELISA References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    FB References

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

    Epitope Map References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    All References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation.
    Schnaar RL
    Biochimica et biophysica acta 1686.3 (2005 Jan 5): 200-8.

    Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections.
    Lauc G
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.8 (2007 Aug): 805-12.

    Differential distribution of major brain gangliosides in the adult mouse central nervous system.
    Heffer M
    PloS one 8.9 (2013): e75720.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    Anti-ganglioside antibodies alter presynaptic release and calcium influx.
    Sheikh KA
    Neurobiology of disease 28.1 (2007 Oct): 113-21.

    Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 127.Pt 5 (2004 May): 1085-100.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review