GT1b-1

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$40.00
SKU: GT1b-1-s
View product citations for antibody GT1b-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Ganglioside GT1b
Depositor: Schnaar, R.L.
Antigen Species: Bovine
Depositors Institution: Johns Hopkins School of Medicine
Isotype: MIgG1
Depositors Notes: GT1b-1 recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Epitope Mapped: Yes
Antigen Molecular Weight: 2.15 kDa
Epitope Location or Sequence: GT1b
Immunogen: KLH-conjugated bovine brain GT1b
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Myeloma Strain: P3x653
Antibody Registry ID: AB_2619573 
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GT1b-1 was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GT1b-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    IF References
    WB References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

    IHC References
    ELISA References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Epitope Map References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    All References

    Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides.
    Shiekh KA
    Analytical biochemistry 302.2 (2002 Mar 15): 276-84.

    Differential distribution of major brain gangliosides in the adult mouse central nervous system.
    Heffer M
    PloS one 8.9 (2013): e75720.

    Biosynthesis of the major brain gangliosides GD1a and GT1b.
    Schnaar RL
    Glycobiology 22.10 (2012 Oct): 1289-301.

    Distribution of mono-, di- and trisialo gangliosides in the brain of Actinopterygian fishes.
    Heffer M
    Biochimica et biophysica acta 1820.9 (2012 Sep): 1437-43.

    Localization of major gangliosides in the PNS: implications for immune neuropathies.
    Sheikh KA
    Brain : a journal of neurology 125.Pt 11 (2002 Nov): 2491-506.

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