H18G8

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$40.00
SKU: H18G8-s
View product citations for antibody H18G8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Interstitial collagenase (MMP-1)
Hybridoma Cells Available: No
Antigen Species: Rabbit
Depositor: Werb, Zena
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California San Francisco
Positive Tested Species Reactivity: Bovine, Canine, Human, Rabbit, Rat
Depositors Notes: This antibody recognizes both proenzyme and active enzyme forms. For paraffin embedded, zinc formalin fixative isrecommended.
Antigen Molecular Weight: Proenzyme:57 & 53 kDa Active Enzyme: 47 & 43 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MMP-1
Immunogen: Partially purified protein from rabbit skin conditioned medium
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-X63-Ag8
Epitope Location or Sequence:
Uniprot ID: P13943 
Immunogen Sequence: Full length protein
Entrez Gene ID: 100009110 
Additional Characterization:  
Antibody Registry ID: AB_528178 
Additional Information: RRID:AB_528178 /For antigen retrieval, use proteinase K.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H18G8 was deposited to the DSHB by Werb, Zena (DSHB Hybridoma Product H18G8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Fibrochondrogenesis of free intraarticular small intestinal submucosa scaffolds.
    Malaviya P
    Tissue engineering 10.1-2 (2004 Jan-Feb): 129-37.

    Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    Damsky CH
    The Journal of cell biology 109.2 (1989 Aug): 877-89.

    Fibrochondrogenesis of free intraarticular small intestinal submucosa scaffolds.
    Malaviya P
    Tissue engineering 10.1-2 (2004 Jan-Feb): 129-37.

    ELISA References
    FFPE References

    Fibrochondrogenesis of free intraarticular small intestinal submucosa scaffolds.
    Malaviya P
    Tissue engineering 10.1-2 (2004 Jan-Feb): 129-37.

    All References

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