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SKU: H36
View product citations for antibody H36 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: p46 cell surface protein excluded from macropinocytic cup
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium
Depositor: Cosson, P.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre Medical Universitaire, Geneva
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 46 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cprG
Immunogen: Crude preparation of Dictyostelium membranes.
Alternate Gene Names: CP7
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63
Epitope Location or Sequence: N-acetylglucosamine-1-phosphate
Uniprot ID: Q94504 
Immunogen Sequence: Full length protein
Entrez Gene ID: 8621915 
Additional Characterization:  
Antibody Registry ID: AB_1553750 
Additional Information: H36 recognizes multiple proteins. The Cystein proteinase 7 (CP7) appears to be the main epitope of H36. H36 is also a marker of multilamellar bodies (MLB) where a different protein CDD (a protein containing a cytidine/deoxycytidylate deaminase (CDD) zinc-binding region) seems to be the target ( see [PMID: 27340834] for more details).
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H36 was deposited to the DSHB by Cosson, P. (DSHB Hybridoma Product H36)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Selective membrane exclusion in phagocytic and macropinocytic cups.
    Cosson P
    Journal of cell science 119.Pt 19 (2006 Oct 1): 4079-87.

    IF References
    WB References
    IP References
    FACS References
    Epitope Map References
    All References

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