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SKU: H36

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DSHB Data Sheet

Catalog Fields

Product Name/ID: H36
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: cprG
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_1553750 
Uniprot ID: Q94504 
Entrez Gene ID: 8621915 
Clonality: Monoclonal
Immunogen: Crude preparation of Dictyostelium membranes.
Immunogen Sequence: Full length protein
Myeloma Strain: X63
Epitope Mapped: Yes
Antigen Name: p46 cell surface protein excluded from macropinocytic cup
Epitope Location or Sequence: N-acetylglucosamine-1-phosphate
Alternate Antigen Name:
Deposit Date: 2/5/2009
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 46 kDa
Depositor: Cosson, P.
Antigen Sequence:
Depositor Institution: Centre Medical Universitaire, Geneva
Antigen Species: Dictyostelium
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Dictyostelium
Additional Information: H36 recognizes multiple proteins. The Cystein proteinase 7 (CP7) appears to be the main epitope of H36. H36 is also a marker of multilamellar bodies (MLB) where a different protein CDD (a protein containing a cytidine/deoxycytidylate deaminase (CDD) zinc-binding region) seems to be the target ( see [PMID: 27340834] for more details).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H36 was deposited to the DSHB by Cosson, P. (DSHB Hybridoma Product H36)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Selective membrane exclusion in phagocytic and macropinocytic cups.
    Cosson P
    Journal of cell science 119.Pt 19 (2006 Oct 1): 4079-87.

    IF References
    WB References
    IP References
    FACS References
    Epitope Map References
    All References

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