H52

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$40.00
SKU: H52
View product citations for antibody H52 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1, kappa light chain
Depositors Institution: Johns Hopkins University School of Medicine
Host Species: mouse
Depositors Notes: Does not cross react with mouse, rat, hamster, rabbit, cow. Blocks leukocyte adhesion and inhibits T cell and NK cell activity. Reactive with frozen sections of human tonsil. Binds all leukocytes.
Positive Tested Species Reactivity: Canine, Human, Pig
Gene: ITGB2
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Immunogen: Human splenic adherent cells .
Clonality: Monoclonal
Alternate Antibody Name: CD18 (beta subunit of CD11a, b, c)
Epitope Mapped: Yes
Myeloma Strain: P3x653-Ag8
Epitope Location or Sequence: Mid-region (aa 362-460)
Uniprot ID: P05107 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3689 
Additional Information: CD18 is a general marker of leukocytes.
Antibody Registry ID: AB_528137 
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H52 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product H52)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IHC References
    IP References
    FB References

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

    Monoclonal and Single Domain Antibodies Targeting β-Integrin Subunits Block Sexual Transmission of HIV-1 in In Vitro and In Vivo Model Systems.
    Markham RB
    Journal of acquired immune deficiency syndromes (1999) 69.3 (2015 Jul 1): 278-85.

    Phospholipase D1 regulates phagocyte adhesion.
    Kusner DJ
    Journal of immunology (Baltimore, Md. : 1950) 176.6 (2006 Mar 15): 3686-96.

    Monoclonal antibodies against porcine LFA-1: species cross-reactivity and functional effects of beta-subunit-specific antibodies.
    Pescovitz MD
    Molecular immunology 26.9 (1989 Sep): 883-95.

    Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation.
    Orentas RJ
    Science (New York, N.Y.) 244.4908 (1989 Jun 2): 1075-8.

    Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes.
    Tedder TF
    Journal of immunology (Baltimore, Md. : 1950) 146.7 (1991 Apr 1): 2235-44.

    Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160.
    Kerr MA
    The Biochemical journal 288 ( Pt 1). (1992 Nov 15): 23-7.

    Biosynthesis and function of membrane bound and secreted forms of recombinant CD11b/CD18 (Mac-1).
    Fong S
    Journal of cellular biochemistry 52.2 (1993 Jun): 183-95.

    FACS References
    Epitope Map References

    The N-terminal region and the mid-region complex of the integrin beta 2 subunit.
    Law SK
    The Journal of biological chemistry 276.39 (2001 Sep 28): 36370-6.

    All References

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

    Monoclonal antibodies against porcine LFA-1: species cross-reactivity and functional effects of beta-subunit-specific antibodies.
    Pescovitz MD
    Molecular immunology 26.9 (1989 Sep): 883-95.

    The role of the cysteine-rich region of the beta2 integrin subunit in the leukocyte function-associated antigen-1 (LFA-1, alphaLbeta2, CD11a/CD18) heterodimer formation and ligand binding.
    Law SK
    FEBS letters 440.3 (1998 Dec 4): 414-8.

    Biosynthesis and function of membrane bound and secreted forms of recombinant CD11b/CD18 (Mac-1).
    Fong S
    Journal of cellular biochemistry 52.2 (1993 Jun): 183-95.

    Monoclonal and Single Domain Antibodies Targeting β-Integrin Subunits Block Sexual Transmission of HIV-1 in In Vitro and In Vivo Model Systems.
    Markham RB
    Journal of acquired immune deficiency syndromes (1999) 69.3 (2015 Jul 1): 278-85.

    Phospholipase D1 regulates phagocyte adhesion.
    Kusner DJ
    Journal of immunology (Baltimore, Md. : 1950) 176.6 (2006 Mar 15): 3686-96.

    Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation.
    Orentas RJ
    Science (New York, N.Y.) 244.4908 (1989 Jun 2): 1075-8.

    Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes.
    Tedder TF
    Journal of immunology (Baltimore, Md. : 1950) 146.7 (1991 Apr 1): 2235-44.

    Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160.
    Kerr MA
    The Biochemical journal 288 ( Pt 1). (1992 Nov 15): 23-7.

    A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.
    Klein N
    The Journal of clinical investigation 103.1 (1999 Jan): 97-106.

    The N-terminal region and the mid-region complex of the integrin beta 2 subunit.
    Law SK
    The Journal of biological chemistry 276.39 (2001 Sep 28): 36370-6.

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