H52
Catalog Fields
Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1, kappa light chain
Depositors Institution: Johns Hopkins University School of Medicine
Host Species: mouse
Depositors Notes: Does not cross react with mouse, rat, hamster, rabbit, cow. Blocks leukocyte adhesion and inhibits T cell and NK cell activity. Reactive with frozen sections of human tonsil. Binds all leukocytes.
Positive Tested Species Reactivity: Canine, Human, Pig
Gene: ITGB2
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Immunogen: Human splenic adherent cells .
Clonality: Monoclonal
Alternate Antibody Name: CD18 (beta subunit of CD11a, b, c)
Epitope Mapped: Yes
Myeloma Strain: P3x653-Ag8
Epitope Location or Sequence: Mid-region (aa 362-460)
Uniprot ID: P05107
Immunogen Sequence: Full length protein
Entrez Gene ID: 3689
Additional Information: CD18 is a general marker of leukocytes.
Antibody Registry ID: AB_528137
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H52 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product H52)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
August JT
Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.