H52
Catalog Fields
Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Hildreth, J.E.K.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Human, Pig
Depositors Institution: Johns Hopkins University School of Medicine
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositors Notes: Does not cross react with mouse, rat, hamster, rabbit, cow. Blocks leukocyte adhesion and inhibits T cell and NK cell activity. Reactive with frozen sections of human tonsil. Binds all leukocytes.
Immunogen: Human splenic adherent cells .
Gene: ITGB2
Alternate Antibody Name: CD18 (beta subunit of CD11a, b, c)
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x653-Ag8
Epitope Mapped: Yes
Uniprot ID: P05107
Epitope Location or Sequence: Mid-region (aa 362-460)
Entrez Gene ID: 3689
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528137
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: CD18 is a general marker of leukocytes.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H52 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product H52)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
August JT
Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.